High-efficiency, large-volume culture flasks allow cells to show strong viability with large expression amount of proteins in the culture of mammalian cells and insect cells. During the culturing process, the use rate of the shaker is significantly increased, and the survival rate and viability of cells are both dramatically elevated. NEST culture flasks also provide high repeatability, which allows highly inter-batch consistency of cell growth and yield. The products have undergone the tightness test, drop test, high-temperature and high-voltage tests, flatness test, tensile test, endotoxin test, sterilization test, DNA/RNA enzyme-free tests and cell culture test, all the results of which meet the corresponding quality standards. Sterile individual package, easy to use.